Production of Helper Dependent Adenovirus Type 5 LRRK2 Expression Vectors
Research Grant, 2016
Study Rationale: † † † † † † † † ††
The LRRK2 gene has been implicated in Parkinsonís disease, but is too large to be expressed in viral vectors, a tool to modify cells. In order to study LRRK2 function, one needs a viral vector that permits expression of LRRK2. Helper dependent adenovirus (HdAd) can package large genes and so are an ideal viral vector system for LRRK2 expression.
The goal of this project is to design HdAd LRRK2 expression vectors that can be used by all Parkinsonís disease researchers.
This project aims to (i) produce HdAd LRRK2 viral vectors, (ii) carry out quality control of produced HdAd vectors, (iii) verify LRRK2 expression, (iv) test vectors in vivo and, subsequently, (v) make vectors available to the Parkinson's disease research community.
Impact on Diagnosis/Treatment of Parkinsonís Disease: † † † † † ††
Currently, researchers in the Parkinsonís disease field are limited in their ability to study LRRK2 function due to the inability to express LRRK2 with viral vectors. Creating an HdAd viral vector that expresses LRRK2 will allow researchers to use this viral vector to help elucidate the mechanisms of LRRK2 function and design relevant therapeutics.
Next Steps for Development:
We expect to generate a battery of LRRK2 viral vectors that all Parkinsonís disease researchers can use in their projects and as a platform to develop novel therapeutics.
Professor in the Department of Neuroscience at University of Florida College of Medicine
Location: Gainesville, Florida, United States
Max Planck Research Group Leader at Max Planck Florida Institute for Neuroscience
Location: Jupiter, Florida, United States